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Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)48 hours refrigerated
Edit Date: 4/19/2010
Performed: Tuesday, Friday
Released: Same day as tested
CPT Code: 86735
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and immediately separate serum from cells. Refrigerate. Freeze at -20° C if specimen cannot be assayed within 48 hours. Allow no more than one freeze/thaw cycle. If multiple tests are ordered, send separate specimen for this test.
Standard Volume: 1 mL serum.
Minimum Volume: 200 µL serum.
Transport: Refrigerated or frozen on dry ice.
Rejection Criteria: Severely lipemic or hemolyzed, icteric, contaminated or heat-inactivated specimen; specimen frozen and thawed more than once; refrigerated specimen received more than 48 hours after collection.

IV=Index Value
<0.9 IV Negative: No significant level of detectable IgG mumps antibody, presumed non-immune to mumps infection.
0.9-1.0 IV Equivocal: Repeat testing in 10-14 days may be helpful.
>or = 1.1 IV Positive: IgG antibody to mumps detected, which may indicate a current or previous exposure or immunization to mumps. Positive IgG antibody levels in the absence of current clinical symptoms may indicate immunity.

PeaceHealth Laboratories