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| 86295:MUMPS VIRUS ANTIBODY, IgM | |||||||||
| Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Monday, Wednesday, Friday | ||||||||
| Released: | 1-6 days after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 86735-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). | ||||||||
| Handling: | Allow to clot, centrifuge and immediately separate serum from cells . Label specimen as ”acute” or ”convalescent.” | ||||||||
| Stability: | 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles. | ||||||||
| Standard Volume: | 1 mL serum. | ||||||||
| Minimum Volume: | 200 µL serum. | ||||||||
| Transport: | Refrigerated. | ||||||||
| Comments: | Parallel testing is preferred and convalescent specimen MUST be received within 30 days from receipt of acute specimen. | ||||||||
| Rejection Criteria: | Severely lipemic, or hemolyzed specimen; heat-inactivated serum; contaminated specimen. | ||||||||
| Reference Range: | |||||||||||||||||||
| IV = Index Value | |||||||||||||||||||
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≤ 0.79 IV – Negative: No significant level of detectable IgM antibody to Mumps virus.
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0.80-1.20 IV – Equivocal: Borderline levels of IgM anti body to Mumps virus. Repeat testing in 10-14 days may be helpful.
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≥ 1.21 IV – Positive: Presence of IgM antibody to Mumps virus detected and may indicate a current or recent infection. However, low levels of IgM anti body may occasionally persist for more than 12 months post-infection or immunization.
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