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Methodology: Enzymatic/Electrophoresis (EP)
Edit Date: 6/11/2010
Performed: Daily
Released: 24-48 hours after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 83625-90 / 83615-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot completely at ambient temperature. Separate serum from cells immediately.
Stability: 7 days ambient. Unstable refrigerated or frozen.
Standard Volume: 1 mL serum.
Minimum Volume: 0.6 mL serum.
Transport: Ambient.
Comments: Do not freeze or refrigerate. Hemolyzed specimens and serum which have not been separated from cells show elevated LD-1 and LD-2. LD-4 and LD-5 are labile at low temperatures and are erroneously low in samples that have been frozen.
Rejection Criteria: Frozen, refrigerated or hemolyzed specimen; specimen collected with EDTA or potassium oxalate/sodium fluoride anticoagulants.

Reference Range:
LD-1: 14-27%
LD-2: 29-42%
LD-3: 16-22%
LD-4: 8-15%
LD-5: 6-23%
Lactate Dehydrogenase, Total:
0 up to 1 mo: 200-465 U/L
1 mo-17 mos: 200-450 U/L
18 mos-10 yrs: 165-430 U/L
11 yrs-16 yrs: 135-310 U/L
17 yrs & over: 105-230 U/L

PeaceHealth Laboratories