 Print View Attached Docs: |
|||||||||
| 57900:GLIADIN ANTIBODIES, IgA & IgG | |||||||||
| Alias Names: | GliadinDP / Gliadin DP / Deamidated Peptide | ||||||||
| Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
| Edit Date: | 4/19/2010 | ||||||||
| Performed: | Monday, Wednesday, Friday | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 83520 x2 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube. | ||||||||
| Handling: | Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial. Store refrigerated until transport. If specimen cannot be assayed within 48 hours, freeze at -20° C. Allow no more than two freeze/thaw cycles. | ||||||||
| Stability: | 459 | ||||||||
| Standard Volume: | 1 mL serum. | ||||||||
| Minimum Volume: | 0.4 mL serum. | ||||||||
| Transport: | Refrigerated or frozen on dry ice. | ||||||||
| Rejection Criteria: | Hyperlipemic, hemolytic, heat treated or contaminated specimen; specimen frozen and thawed more that twice; refrigerated specimen received in laboratory more than 48 hours after collection. | ||||||||
| Gliadin IgG and IgA Antibody Result Interpretations, ELISA Method: | |||||||||||||||||||
|
<20 EU |
|||||||||||||||||||
| Negative | <20 EU | ||||||||||||||||||
| Weak Positive | 20-30 EU | ||||||||||||||||||
| Positive | >30 EU | ||||||||||||||||||
|
EU = Elisa Units Glaidins are a group of proteins found in the gluten of wheat and rye grains. In susceptible individuals, alpha-gliadins are known to be necessary for the activation of celiac disease. Gliadin antibodies are useful in screening populations at risk for gluten sensitive disease (celiac disease, dermatitis herpetiformis) and in monitoring compliance to gluten-free diet. |
|||||||||||||||||||