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| 27500:CARDIOLIPIN ANTIBODIES, IgG & IgM | |||||||||
| Alias Names: | Anti-Cardiolipin Antibodies / Anti-Phospholipid Antibodies | ||||||||
| Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
| Edit Date: | 4/19/2010 | ||||||||
| Performed: | Monday, Thursday | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 86147 x2 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 5 mL red top tube. Also acceptable: One 7.5 mL serum separator tube (SST). | ||||||||
| Handling: | Allow to clot, centrifuge and separate serum from cells immediately and pour into an aliquot tube. Refrigerate. Freeze at -20° C if specimen can not be assayed within 48 hours. | ||||||||
| Standard Volume: | 2 mL serum. | ||||||||
| Minimum Volume: | 0.5 mL serum. | ||||||||
| Transport: | Refrigerated or frozen on dry ice. | ||||||||
| Comments: | See also: Coagulation Study, Anti-Phospholipid Panel (25620) . | ||||||||
| Rejection Criteria: | Refrigerated specimen received in laboratory more than 48 hours after collection; plasma; microbially contaminated, heat-treated, grossly hemolyzed or lipemic specimen. | ||||||||
| Reference Ranges: | |||||||||||||||||||
| Result Interpretations, ELISA Method: | |||||||||||||||||||
| Cardiolipin, IgG | <16 GPL | Normal | |||||||||||||||||
| 17-25 GPL | Borderline | ||||||||||||||||||
| 26-79 GPL | Positive | ||||||||||||||||||
| ≥ 80 GPL | High Positive | ||||||||||||||||||
| Cardiolipin, IgM | <12 MPL | Normal | |||||||||||||||||
| 12-19 MPL | Borderline | ||||||||||||||||||
| 20-79 MPL | Positive | ||||||||||||||||||
| ≥ 80 MPL | High Positive | ||||||||||||||||||
| GPL = IgG Phospholipid Units | |||||||||||||||||||
| MPL = IgM Phospholipid Units | |||||||||||||||||||
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Studies suggest that the higher the IgG anticardiolipin antibody titer, the greater the clinical significance. Values greater than 40 may be the most significant based on recent studies, however an absolute threshold has not been clearly established. Results in the borderline range and isolated elevations of IgM are of uncertain clinical significance and must be carefully interpreted with regard to the clinical picture. Transient positive results occur, usually in association with infection. Demonstrated persistence of positive lab findings (retest after 8 weeks) is required for the lab diagnosis of anti-phospholipid syndrome. Other phospholipid-directed antibodies (e.g., anti-beta2 glycoprotein1, Beta2 Glycoprotein1 Antibodies IgG & IgM, 86990) may be useful in some patients. |
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