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|60270:BLOOD CULTURE, BRUCELLA|
|Methodology:||BacT/Alert Continuous Monitoring Bacterial System|
|Released:||Preliminary at 24 hours or as soon as positive is detected. Final at 28 days.|
|Specimen Collection Details|
|Collection:||Draw blood into BacT/Alert FA (aerobic) and/or FN (anaerobic), or PF (pediatric) bottles using aseptic technique as defined for blood culture collection. Bottle(s) are to be at ambient temperature. Do not refrigerate or freeze. Inspect broth and sensor and do not use if broth is cloudy or sensor is yellow. Recommended volumes to collect per draw:
NEONATES TO ONE YEAR: 0.5 to 1.5 mL in a BacT/Alert PF bottle (1.0 mL minimum preferred).
CHILDREN 1 TO 6 YEARS: 0.5 mL per year of age in a BacT/Alert PF bottle.
CHILDREN WEIGHING 30-80 POUNDS: 4-10 mL. If 4 mL or less is collected, use the BacT/Alert PF bottle. If between 4 and 10 mL is collected, use the BacT/Alert FA blood culture bottle. If 10 mL is collected, divide between the BacT/Alert FA and the BacT/Alert FN bottles.
CHILDREN WEIGHING MORE THAN 80 POUNDS AND ADULTS: 20 mL divided between the BacT/Alert FA and BacT/Alert FN bottles. Occasionally it will not be possible to collect 20 mL per draw from a patient in this group. In that case, inoculate the bottle(s) as described above for children weighing 30-80 pounds.
|Stability:||24 hours ambient. Do not refrigerate. Unstable frozen.|
|Standard Volume:||BacT/Alert FA and FN bottles: 10 mL blood each. BacT/Alert PF bottles: 4 mL blood.|
|Minimum Volume:||BacT/Alert FA and FN bottles: 5 mL blood each. BacT/Alert PF bottles: 1 mL blood.|
|Comments:||Isolated organisms will be identified. Susceptibility testing will be done if appropriate. An additional charge will be added for these tests.|
|Rejection Criteria:||Frozen specimen.|
|No growth at 3 weeks.|