Anti-DNA Antibody, Single Stranded / Anti-ssDNA Antibldy, IgG
Methodology:
Enzyme-Linked Immunossorbent Assay (ELISA)
Edit Date:
6/11/2010
Performed:
Tuesday, Thursday, Saturday.
Released:
1-4 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code:
86226-90
Specimen Collection Details
Collection:
One 7.5 mL serum separator tube (SST).
Handling:
Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial.
Stability:
48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
Standard Volume:
1 mL serum
Minimum Volume:
100 µL serum
Transport:
Refrigerated
Comments:
The ssDNA antibody test detects ssDNA and some dsDNA antibodies. As a result, patient samples should also be tested for dsDNA antibodies in order to more accurately determine the level of anti-ssDNA antibodies. By calculating the ratio (ssDNA/dsDNA), a conclusion can be made as to the presence of ssDNA antibodies. A ratio less than or equal to 1.0 indicates the absence of ssDNA antibodies. A ratio greater than 1.0 indicates the presence of ssDNA antibodies.
Rejection Criteria:
Plasma; severly lipemic, contaminated, or hemolyzed specimen.
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