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| 57910:TISSUE TRANSGLUTAMINASE (TTG) ANTIBODY, IgA | |||||||||
| Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
| Edit Date: | 4/19/2010 | ||||||||
| Performed: | Monday, Wednesday, Friday | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 83520 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube. | ||||||||
| Handling: | Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial. Store refrigerated until transport. If specimen cannot be assayed within 48 hours, freeze at -20° C. Allow no more than two freeze/thaw cycles. | ||||||||
| Standard Volume: | 1 mL serum. | ||||||||
| Minimum Volume: | 0.4 mL serum. | ||||||||
| Transport: | Refrigerated or frozen on dry ice. | ||||||||
| Rejection Criteria: | Hyperlipemic, hemolytic, heat-treated, or contaminated specimen; specimen frozen and thawed more than twice; refrigerated specimen received in laboratory more than 48 hours after collection. | ||||||||
| Reference Range: | |||||||||||||||||||
| <20 ELISA Units (EU) | |||||||||||||||||||
| Result Interpretations, ELISA method: | <20 EU | None Detected | |||||||||||||||||
| 20-30 EU | Weak Positive | ||||||||||||||||||
| >30 EU | Positive | ||||||||||||||||||
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The endomysial antigen has been identified as the protein cross-linking enzyme known as tissue transglutaminase. Detection of tissue transglutaminase antibodies is an aid in the diagnosis of certain gluten-sensitive diseases, such as Celiac disease and dermatitis herpetiformis. |
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