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Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 4/19/2010
Performed: Monday, Wednesday, Friday
Released: Same day as tested
CPT Code: 83520
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial. Store refrigerated until transport. If specimen cannot be assayed within 48 hours, freeze at -20° C. Allow no more than two freeze/thaw cycles.
Standard Volume: 1 mL serum.
Minimum Volume: 0.4 mL serum.
Transport: Refrigerated or frozen on dry ice.
Rejection Criteria: Hyperlipemic, hemolytic, heat-treated, or contaminated specimen; specimen frozen and thawed more than twice; refrigerated specimen received in laboratory more than 48 hours after collection.

Reference Range:
<20 ELISA Units (EU)
Result Interpretations, ELISA method: <20 EU None Detected
20-30 EU Weak Positive
>30 EU Positive

The endomysial antigen has been identified as the protein cross-linking enzyme known as tissue transglutaminase. Detection of tissue transglutaminase antibodies is an aid in the diagnosis of certain gluten-sensitive diseases, such as Celiac disease and dermatitis herpetiformis.

PeaceHealth Laboratories