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Alias Names: Antiendomysial
Methodology: Indirect Immunofluorescent Antibody (IFA)
Edit Date: 4/19/2010
Performed: Monday, Wednesday, Friday
Released: Same day as tested
CPT Code: 86256
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and separate serum from cells and pour into a plastic vial. Store refrigerated until transport. If specimen cannot be assayed within 48 hours, freeze at -20° C. Allow no more than two freeze/thaw cycles.
Standard Volume: 1 mL serum.
Minimum Volume: 0.4 mL serum.
Transport: Refrigerated or frozen on dry ice.
Rejection Criteria: Hyperlipemic; hemolytic; heat treated or contaminated samples; specimen received in laboratory more than 48 hours after collection; specimen frozen/thawed more than twice.

<1_5 titer
Endomysial Antibody, IgA Result Interpretation, IFA Method less than 1:5 titer greater or equal to 1:5 titer
Negative: No significant level of detectable Endomysial IgA antibody Positive: Endomysial IgA antibody detected

IgA class Endomysial Antibodies are seen in gluten-sensitive enteropathies. Approximately 70% of patients with dermatitis herpetiformis and almost 100% of patients with active Celiac disease will demonstrate IgA antibodies. Antibody levels fall with gluten-free diets and reappear upon gluten challenge.

PeaceHealth Laboratories