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69850:ENDOMYSIAL ANTIBODY, IgA | |||||||||
Alias Names: | Antiendomysial | ||||||||
Methodology: | Indirect Immunofluorescent Antibody (IFA) | ||||||||
Edit Date: | 4/19/2010 | ||||||||
Performed: | Monday, Wednesday, Friday | ||||||||
Released: | Same day as tested | ||||||||
CPT Code: | 86256 | ||||||||
Specimen Collection Details | |||||||||
Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube. | ||||||||
Handling: | Allow to clot, centrifuge and separate serum from cells and pour into a plastic vial. Store refrigerated until transport. If specimen cannot be assayed within 48 hours, freeze at -20° C. Allow no more than two freeze/thaw cycles. | ||||||||
Standard Volume: | 1 mL serum. | ||||||||
Minimum Volume: | 0.4 mL serum. | ||||||||
Transport: | Refrigerated or frozen on dry ice. | ||||||||
Rejection Criteria: | Hyperlipemic; hemolytic; heat treated or contaminated samples; specimen received in laboratory more than 48 hours after collection; specimen frozen/thawed more than twice. |
<1_5 titer | |||||||||||||||||||
Endomysial Antibody, IgA Result Interpretation, IFA Method | less than 1:5 titer | greater or equal to 1:5 titer | |||||||||||||||||
Negative: No significant level of detectable Endomysial IgA antibody | Positive: Endomysial IgA antibody detected | ||||||||||||||||||
IgA class Endomysial Antibodies are seen in gluten-sensitive enteropathies. Approximately 70% of patients with dermatitis herpetiformis and almost 100% of patients with active Celiac disease will demonstrate IgA antibodies. Antibody levels fall with gluten-free diets and reappear upon gluten challenge. |