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80852:MYCOPLASMA PNEUMONIAE ANTIBODY, IgG & IgM | |||||||||
Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
Edit Date: | 6/11/2010 | ||||||||
Performed: | Daily | ||||||||
Released: | 24-72 hours after setup at PeaceHealth Laboratories’ reference lab, next run if titered. | ||||||||
CPT Code: | 86738-90 x2 | ||||||||
Specimen Collection Details | |||||||||
Collection: | One 7.5 mL serum separator tube (SST). | ||||||||
Handling: | Allow to clot, centrifuge and separate serum from cells and immediately pour into a plastic vial. Refrigerate. Specimens must be labeled ‘acute’ or ‘convalescent’. | ||||||||
Stability: | 48 hours ambient, 14 days refrigerated, or 1 year frozen. | ||||||||
Standard Volume: | 0.5 mL serum. | ||||||||
Minimum Volume: | 100 µL serum. | ||||||||
Transport: | Refrigerated. | ||||||||
Comments: | Parallel testing is preferred and convalescent specimen must be received within 30 days of the receipt of acute specimen. | ||||||||
Rejection Criteria: | Plasma; severly lipemic, hemolyzed, heat-inactivated, contaminated, or icteric specimen. |
Reference Range: | |||||||||||||||||||
Mycoplasma pneumoniae Antibody, IgG | <0.10 U/L | Negative | |||||||||||||||||
0.10-0.32 U/L | Equivocal | ||||||||||||||||||
>0.32 /L | Positive | ||||||||||||||||||
Mycoplasma pneumoniae Antibody, IgM | ≤0.76 U/L | Negative. No clinically significant amount of M. pneumoniae antibody detected. | |||||||||||||||||
0.77-0.95 U/L | Low positive. M. pneumoniae-specific IgM presumptively detected. Collection of a follow-up sample in one to two weeks is recommended to assure reactivity. | ||||||||||||||||||
≥0.96 U/L | Positive. Highly significant amount of M. pneumoniae-specific IgM antibody detected. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection. | ||||||||||||||||||