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| 82700:CORTISOL, URINARY FREE, 24-HOUR URINE | |||||||||
| Alias Names: | Urinary Free Cortisol | ||||||||
| Methodology: | Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Daily | ||||||||
| Released: | 24-48 hours after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 82530-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | 24-hour urine in a clean, dry plastic container. Refrigerate during collection and handling. Also acceptable: random urine. | ||||||||
| Handling: | Note total volume and time collection started and stopped. | ||||||||
| Stability: | 48 hours ambient, 14 days refrigerated, or 6 months frozen. | ||||||||
| Standard Volume: | 3 mL aliquot. | ||||||||
| Minimum Volume: | 1 mL aliquot. | ||||||||
| Transport: | Frozen on dry ice, or refrigerated at 2-8° C. | ||||||||
| Comments: | No dietary restirctions besides excessive Prednisolone medication. | ||||||||
| Rejection Criteria: | Specimen that has been preserved or acidified. | ||||||||
| Test Name | Age | Female | Male | ||||||||||||||||
| Cortisol (µg/day) | 3-8 years | <18 µg/day | <18 µg/day | ||||||||||||||||
| 9-12 years | <37 µg/day | <37 µg/day | |||||||||||||||||
| 13-17 years | <56 µg/day | <56 µg/day | |||||||||||||||||
| ≥ 18 years | <45 µg/day | <60 µg/day | |||||||||||||||||
| Cortisol (µg/g crt) | Prepubertal | <25 µg/g crt | <25 µg/g crt | ||||||||||||||||
| ≥ 18 years | <45 µg/g crt | <32 µg/g crt | |||||||||||||||||
| Pregnancy | <59 µg/g crt | ||||||||||||||||||
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Ratios to creatinine may be useful for evaluation when the urine collection is random, other than 24-hour, or the urine volume is less than 400 mL/24 hours. Low urinary cortisol concentrations may be consistent with adrenal insufficiency. |
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