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Methodology: Chemiluminescent (CLIA)
Edit Date: 4/18/2010
Performed: Daily
Released: Same day as tested
CPT Code: 84402
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube. Collect at least 4 mL. Draw specimen between 0600 and 1000 if possible, as testosterone levels vary throughout the day.
Handling: Allow to clot, centrifuge and separate serum from cells within 2 hours. Refrigerate. Freeze if specimen cannot be assayed within 7 days. Allow no more than one freeze/thaw cycle.
Stability: 7 days refrigerated at 2-8° C, or 2 months frozen at -20° C.
Standard Volume: 2 mL serum.
Minimum Volume: 1 mL serum.
Transport: Refrigerated, or frozen on dry ice.
Comments: Grossly lipemic specimen may yield falsely elevated values.

Free Testosterone is useful in screening for hirsutism.

Rejection Criteria: Gross hemolysis; gross lipemia, unless cleared by ultracentrifugation; specimen frozen and thawed more than once.

Reference Range:
Age Male Age Female
1-9 years <1.0 pg/mL 1-9 years <1.8 pg/mL
10-11 years 0.1-6.3 pg/mL 10-11 years 0.1-3.5 pg/mL
12-13 years 0.5-98 pg/mL 12-13 years 0.9-6.8 pg/mL
14-15 years 3.0-138 pg/mL 14-15 years 1.2-7.5 pg/mL
16-17 years 38-173 pg/mL 16-17 years 1.2-9.9 pg/mL
≥18 years 47-244 pg/mL 18-30 years 0.8-7.4 pg/mL
31-40 years 1.3-9.2 pg/mL
41-51 years 1.1-5.8 pg/mL
Postmenopausal 0.6-3.8 pg/mL
Tanner Stage I ≥3.7 pg/mL Tanner Stage I <2.2 pg/mL
Tanner Stage II 0.3-21 pg/mL Tanner Stage II 0.4-4.5 pg/mL
Tanner Stage III 1.0-98 pg/mL Tanner Stage III 1.3-7.5 pg/mL
Tanner Stage IV 35-169 pg/mL Tanner Stage IV 1.1-15.5 pg/mL
Tanner Stage V 41-239 pg/mL Tanner Stage V 0.8-9.2 pg/mL

PeaceHealth Laboratories