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| 40553:TESTOSTERONE, FREE | |||||||||
| Methodology: | Chemiluminescent (CLIA) | ||||||||
| Edit Date: | 4/18/2010 | ||||||||
| Performed: | Daily | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 84402 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube. Collect at least 4 mL. Draw specimen between 0600 and 1000 if possible, as testosterone levels vary throughout the day. | ||||||||
| Handling: | Allow to clot, centrifuge and separate serum from cells within 2 hours. Refrigerate. Freeze if specimen cannot be assayed within 7 days. Allow no more than one freeze/thaw cycle. | ||||||||
| Stability: | 7 days refrigerated at 2-8° C, or 2 months frozen at -20° C. | ||||||||
| Standard Volume: | 2 mL serum. | ||||||||
| Minimum Volume: | 1 mL serum. | ||||||||
| Transport: | Refrigerated, or frozen on dry ice. | ||||||||
| Comments: | Grossly lipemic specimen may yield falsely elevated values.
Free Testosterone is useful in screening for hirsutism. |
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| Rejection Criteria: | Gross hemolysis; gross lipemia, unless cleared by ultracentrifugation; specimen frozen and thawed more than once. | ||||||||
| Reference Range: | |||||||||||||||||||
| Age | Male | Age | Female | ||||||||||||||||
| 1-9 years | <1.0 pg/mL | 1-9 years | <1.8 pg/mL | ||||||||||||||||
| 10-11 years | 0.1-6.3 pg/mL | 10-11 years | 0.1-3.5 pg/mL | ||||||||||||||||
| 12-13 years | 0.5-98 pg/mL | 12-13 years | 0.9-6.8 pg/mL | ||||||||||||||||
| 14-15 years | 3.0-138 pg/mL | 14-15 years | 1.2-7.5 pg/mL | ||||||||||||||||
| 16-17 years | 38-173 pg/mL | 16-17 years | 1.2-9.9 pg/mL | ||||||||||||||||
| ≥18 years | 47-244 pg/mL | 18-30 years | 0.8-7.4 pg/mL | ||||||||||||||||
| 31-40 years | 1.3-9.2 pg/mL | ||||||||||||||||||
| 41-51 years | 1.1-5.8 pg/mL | ||||||||||||||||||
| Postmenopausal | 0.6-3.8 pg/mL | ||||||||||||||||||
| Tanner Stage I | ≥3.7 pg/mL | Tanner Stage I | <2.2 pg/mL | ||||||||||||||||
| Tanner Stage II | 0.3-21 pg/mL | Tanner Stage II | 0.4-4.5 pg/mL | ||||||||||||||||
| Tanner Stage III | 1.0-98 pg/mL | Tanner Stage III | 1.3-7.5 pg/mL | ||||||||||||||||
| Tanner Stage IV | 35-169 pg/mL | Tanner Stage IV | 1.1-15.5 pg/mL | ||||||||||||||||
| Tanner Stage V | 41-239 pg/mL | Tanner Stage V | 0.8-9.2 pg/mL | ||||||||||||||||