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Methodology: Radial Immunodiffusion (RID)
Edit Date: 4/20/2010
Performed: Daily
Released: 48 hours
CPT Code: 85385
Specimen Collection Details
Collection: One 4.5 mL light blue top tube (sodium citrate). Collect by careful venipuncture to avoid tissue fluid contamination or hemolysis. Do not use needles smaller than 23 gauge. Fill tube to maximum draw (at least 90% full). Mix by gentle inversion.
Handling: Refrigerate and keep capped in original tube if specimen will be received in the laboratory within 4 hours. If not, centrifuge and separate plasma. Refrigerate. Label tube with patient name, test, and “plasma.”
Stability: Separated plasma stable 48 hours refrigerated or 14 days frozen.
Standard Volume: 5 mL whole blood or 2 mL plasma.
Minimum Volume: 1.8 mL whole blood or 0.5 plasma.
Transport: Refrigerated specimen in original tubes if received in laboratory within 4 hours of collection, or plasma frozen dry ice.
Comments: For routine fibrinogen testing order Fibrinogen (25200). The antigenic test is used to evaluate patients with low functional fibrinogen levels for dysfibrinogenemia.
Rejection Criteria: Serum; visible fibrin; moderate to marked hemolysis; specimen in original tube received in laboratory more than 4 hours after collection.

PeaceHealth Laboratories