84334:HERPES SIMPLEX VIRUS (HSV) CULTURE REFLEX TO TYPING
Alias Names:
HSV CULTURE REFLEX TO TYPING
Methodology:
Enzyme Linked Virus Inducible System (ELVIS)
Edit Date:
2/7/2007
Performed:
Monday-Saturday
Released:
5-7 days after setup at PeaceHealth Laboratories’ reference lab. If positive, add 2 additional days.
CPT Code:
87255-90 Culture; 87300-90 Typing, if positive
Specimen Collection Details
Collection:
Genital lesions, mouth swab, vesicle swab or fluid. Place swab or specimen into MicroTest Transport Media (M4). Also acceptable: Cerebral spinal fluid (CSF) in sterile leak proof container. Do not dilute CSF in media (M4). Source of specimen is required.
Handling:
Freeze at –70° C or on dry ice. Do not freeze specimen at –20° C as virus loses infectivity.
Stability:
24 hours ambient, 72 hours refrigerated, or 2 months frozen.
Transport:
Frozen on dry ice.
Comments:
Specimens should be collected early in the acute phase of infection. The chance of viral recovery is best during the first 3 days after onset and is greatly reduced beyond 5 days with many viruses. Assay detects and identifies HSV using “transgenic cell technology.” HSV is responsible for several clinically significant human viral diseases, with severity ranging from mild to fatal. HSV Type 1 infections are usually found above the waist. HSV Type 2 infections are more commonly seen in association with the genitalia and surrounding areas and are usually sexually transmitted. It is important to note, however, that both HSV Type 1 and 2 have been involved in all disease manifestations and locations of the body. Positives will be typed.
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