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Methodology: Ion Exchange Chromatography (IEC)
Edit Date: 3/12/2008
Performed: Monday
Released: 4-11 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 83505-90
Specimen Collection Details
Collection: 24-hour urine. Refrigerate during collection. Patient should be on a collagen-free diet for 3 days prior to collection.
Handling: Record total volume and collection time. Mix well and pour off a 10 mL aliquot and freeze immediately after collection.
Stability: 48 hours refrigerated or 6 months frozen. Unstable ambient.
Standard Volume: 10 mL urine.
Minimum Volume: 3 mL urine.
Transport: Frozen on dry ice.
Rejection Criteria: Non-frozen specimen.

Reference Range
Hydroxyproline, Total 38500 umol/d
Age Male Female
Creatinine (24 hour) 18-80 years 1000-2500 mg/d 700-1600 mg/d
51-80 years 800-2100 mg/d 500-1400 mg/d
>80 years 600-2000 mg/d 400-1300 mg/d

PeaceHealth Laboratories