One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling:
Allow to clot, centrifuge and separate serum from cells immediately. Refrigerate. Freeze at-20º C if specimen cannot be assayed within 7 days. Allow no more than three freeze/thaw cycles. If multiple tests are ordered, send separate specimen for this test.
Standard Volume:
1 mL serum.
Minimum Volume:
0.5 mL serum.
Transport:
Refrigerated, or frozen on dry ice.
Rejection Criteria:
Plasma; grossly contaminated, hemolyzed, lipemic, heat-inactivated, or icteric specimen; specimen received in laboratory more than 7 days after collection; specimen frozen and thawed more than three times.
Reference Range: <0.9 IA (IA = Antiibody Index)
Herpes Simplex Virus (HSV) Type 2 Glycoprotein G-Specific Antibody, IgG
<0.9 IA
Negative: No significant level of detectable IgG antibody to HSV type 2 glycoprotein G.
0.9-1.0 IA
Equivocal: Questionable presence of IgG antibody to HSV type 2. Repeat testing in 10-14 days may be helpful.
≥ 1.1 AI
Positive: IgG antibody to HSV type 2 glycoprotein G detected, which may indicate a current or recent HSV infection.
Individuals infected with HSV may not exhibit detectable IgG antibody to glycoprotein G in the early stages of infection and 5-10% of infections may occur with glycoprotein G deficient virus. Detection of antibody presence in these cases may only be possible using a non-type-specific screening test.
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