81222:STRIATED MUSCLE ANTIBODY, IgG WITH REFLEX TO TITER
Methodology:
Indirect Fluorescent Antibody (IFA)
Edit Date:
6/11/2010
Performed:
Monday-Friday
Released:
1-5 days after setup at PeaceHealth Laboratories’ reference lab (48 hours if titered).
CPT Code:
86255-90, if reflexed add 86256-90
Specimen Collection Details
Collection:
One 7.5 mL serum separator tube (SST).
Handling:
Allow to clot, centrifuge and separate serum from cells immediately and pour into a plastic vial.
Stability:
48 hours ambient, 2 weeks refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
Standard Volume:
1 mL serum.
Minimum Volume:
150 µL serum.
Transport:
Refrigerated.
Comments:
Titers greater than or equal to 1:80 are suggestive of myasthenia; however, striated muscle antibody can be found in rheumatic fever, myocardial infarction, and a variety of post-cardiotomy states. All positives will be titered to endpoint. If Striated Muscle Antibody is >1:40, a titer will be added.
Rejection Criteria:
Plasma; grossly lipemic, hemolyzed, or contaminated specimen.
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