42156:HUMAN IMMUNODEFICIENCY VIRUS-1 (HIV-1) ANTIBODY, RAPID; REFLEX by WESTERN BLOT
Methodology:
Immunochromatographic Assay (ICT)
Edit Date:
2/14/2008
Performed:
Daily
Released:
Same day
CPT Code:
86701
Specimen Collection Details
Collection:
One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube or one 4 mL lavender top tube.
Handling:
Allow to clot, centrifuge and separate serum or plasma from cells immediately and pour into a plastic vial. Refrigerate. Freeze if specimen cannot be assayed within 5 days. Avoid multiple freeze/thaw cycles.
Stability:
Specimen stable 5 days refrigerated or indefinitely frozen.
Standard Volume:
1 mL serum or plasma.
Minimum Volume:
0.3 mL serum or plasma.
Transport:
Refrigerated, or if frozen, on dry ice.
Comments:
CDC recommendation includes that all reactive rapid HIV-1 antibody test results must be confirmed with HIV Western Blot. Testing for rapid HIV-1 Antibody can also be performed on whole blood with confirmation by Western Blot, however, it must be performed on serum or plasma.
Rejection Criteria:
Gross hemolysis or lipemia; multiple freeze/thaw cycles.
MEDICARE MAY REQUIRE A SIGNED ADVANCE BENEFICIARY NOTICE (ABN) FOR THIS TEST
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