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Methodology: Serum Neutralization Assay
Edit Date: 11/17/2010
Performed: Monday-Saturday
Released: 6-9 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 86658-90 x3
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: Cerebrospinal spinal fluid (CSF).
Handling: Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial. CSF in a sterile container. Refrigerate.
Stability: 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
Standard Volume: 1 mL serum or CSF.
Minimum Volume: 0.3 mL serum or CSF.
Transport: Refrigerated.
Comments: The best indication of immunity to poliovirus is neutralization.
Rejection Criteria: Plasma.

No significant level of detectable poliovirus antibodies.
≥ 1:10
Antibody to poliovirus detected, which may represent prior immunization or current or past infection.

In immunized individuals, the significance of a low antibody titer to poliovirus 3 (the least immunogenic vaccine serotype) is unclear.

Interpretive Data: The presence of poliovirus antibodies may represent prior immunization or acute infection. The clinical significance of and the criteria for interpretation of results may require consultation with and Infectious Disease Specialist.

PeaceHealth Laboratories