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Alias Names: Streptococcus pneumoniae Immune Response / Streptococcus pneumoniae Vaccine Response
Methodology: Multi-Analyte Fluorescent Detection
Edit Date: 6/11/2010
Components: Includes serotypes 1, 3, 4*, 5, 6B*, 7F, 8, 9N, 9V*, 12F, 14*, 18C*, 19F*, 23F*
All sesrotypes tested are present in the 23-valent pure polysaccharide pneumococcal vaccine. The serotypes marked with an asterisk are contained in the heptavalent conjugated pneumococcal vaccine.
Performed: Monday, Thursday, Saturday
Released: 1-4 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 86317-90 x14
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and separate serum from cells immediately. Label specimen ‘Pre-Vaccine’ or ‘Post-Vaccine.’
Stability: After separation from clot, specimen stable 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
Standard Volume: 1.5 mL serum.
Minimum Volume: 250 µL serum.
Transport: Refrigerated.
Comments: Pre- and post-pneumococcal vaccine specimens can be submitted separately or together for testing. Post- specimen should be drawn 30 days after immunization and, if shipped separately, must be received within 60 days of the pre-specimen. Label specimens clearly with ‘Pre-Vaccine’ or ‘Post-Vaccine.’
Rejection Criteria: Plasma; other body fluids; severely lipemic, contaminated or hemolyzed specimen.

Reference Interval:
This assay is designed to use both ‘pre’ and ‘post’ specimens to assess immune responsiveness to vaccination. This test is not designed to determine protection to Streptococcus pneumoniae based on a single specimen.
Long-term protection is generally thought to be associated with a one month post-vaccine response of at least 1 µg/mL in children and adults. Responder status is determined once reaching the minimum level of 1 µg/mL, according to the ratio of post-vaccination to pre-vaccination concentration of pneumococcal IgG antibody, as follows.
A ratio of less than twofold is considered a non-responder.
A ratio of two-to fourfold is a weak responder.
A ratio of fourfold or greater is a good responder.

PeaceHealth Laboratories