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| 29875:PAROXYSMAL NOCTURNAL HEMOGLOBINURIA (PNH) STUDY, RBC and GRANULOCYTE. | |||||||||
| Alias Names: | Hemolysins / HAM test / PNH / CD55 AND CD59 / | ||||||||
| Methodology: | Flow Cytometry (FC) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Daily | ||||||||
| Released: | Next day | ||||||||
| CPT Code: | 88184 / 88185 x4 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 4 mL lavender top tube (EDTA). | ||||||||
| Handling: | Submit whole blood at ambient temperature. Specimen must arrive at laboratory within 24 hours of collection. | ||||||||
| Stability: | 24 hours ambient. | ||||||||
| Standard Volume: | 4 mL whole blood. | ||||||||
| Minimum Volume: | 1 mL whole blood. | ||||||||
| Transport: | Ambient. | ||||||||
| Comments: | The underlying defect in paroxysmal nocturnal hemoglobinuria (PNH) has been traced to a gene mutation which codes for a GPI anchor protein which binds complement-regulating proteins to the red cell surface. Absence of GPI anchored proteins such as CD55 and CD59 is a highly specific indication of presence of the PNH defect. | ||||||||
| Rejection Criteria: | Clotted, refrigerated or frozen specimen; tubes other than specified; specimen received in laboratory more than 24 hours after collection. | ||||||||
| Reference Range: | |||||||||||||||||||
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<1.0% RBCs showing PNH phenotype
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<0.01% Granulocytes showing PNH phenotype
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