29875:PAROXYSMAL NOCTURNAL HEMOGLOBINURIA (PNH) STUDY, RBC and GRANULOCYTE.

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29875:PAROXYSMAL NOCTURNAL HEMOGLOBINURIA (PNH) STUDY, RBC and GRANULOCYTE.
Alias Names: Hemolysins / HAM test / PNH / CD55 AND CD59 /
Methodology: Flow Cytometry (FC)
Edit Date: 6/11/2010
Performed: Daily
Released: Next day
CPT Code: 88184 / 88185 x4
Specimen Collection Details
Collection: One 4 mL lavender top tube (EDTA).
Handling: Submit whole blood at ambient temperature. Specimen must arrive at laboratory within 24 hours of collection.
Stability: 24 hours ambient.
Standard Volume: 4 mL whole blood.
Minimum Volume: 1 mL whole blood.
Transport: Ambient.
Comments: The underlying defect in paroxysmal nocturnal hemoglobinuria (PNH) has been traced to a gene mutation which codes for a GPI anchor protein which binds complement-regulating proteins to the red cell surface. Absence of GPI anchored proteins such as CD55 and CD59 is a highly specific indication of presence of the PNH defect.
Rejection Criteria: Clotted, refrigerated or frozen specimen; tubes other than specified; specimen received in laboratory more than 24 hours after collection.
MEDICARE MAY REQUIRE A SIGNED ADVANCE BENEFICIARY NOTICE (ABN) FOR THIS TEST

Reference Range:
<1.0% RBCs showing PNH phenotype
<0.01% Granulocytes showing PNH phenotype

PeaceHealth Laboratories