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Methodology: Estradiol: Chemiluminescence (CL); Estrone: Radioimmunoassay (RIA)
Edit Date: 6/7/2008
Performed: Estradiol: Daily; Estrone: Monday
Released: 1-7 days
CPT Code: 82671
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and separate serum from cells immediately. Divide serum between two separate plastic tubes. Refrigerate. Freeze if specimens will not be assayed within 24 hours.
Standard Volume: 1 mL serum.
Minimum Volume: 0.5 mL serum.
Transport: Refrigerated if received in laboratory within 24 hours of collection, or frozen on dry ice.
Rejection Criteria: Gross hemolysis or gross lipemia; refrigerated specimen received in laboratory more than 24 hours after collection.

Estradiol – Female Early Follicular <160 pg/mL
Late Follicular 34-400 pg/mL
Luteal 27-246 pg/mL
Postmenopausal (untreated) <30 pg/mL
Postmenopausal (treated) <93 pg/mL
Oral Contraceptive <102 pg/mL
Estradiol – Male <56 pg/mL
Estrone – Female Early Follicular <150 pg/mL
Late Follicular 100-250 pg/mL
Luteal <200 pg/mL
Postmenopausal <55 pg/mL
Prepubertal <25 pg/mL
Estrone-Male Adult <65 pg/mL
Prepubertal <25 pg/mL

PeaceHealth Laboratories