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Methodology: Broth Dilution (Schlichter)
Edit Date: 6/11/2010
Performed: Daily
Released: Within 6 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 87197-90
Specimen Collection Details
Collection: Both peak and trough blood or body fluid specimens are collected. For each serum specimen collect one 7.5 mL serum separator tube (SST) using sterile technique. Collect body fluid in a sterile container.
Handling: After blood is centrifuged, transfer serum to a sterile tube using sterile technique. Label as ‘peak’ or ‘trough’, if known. Test requires either serum or body fluid and an organism that was previously cultured from the patient. Obtain specified organism from the Microbiology Department. The following information is required: time and date of collection, diagnosis, time of last dose, and list of all antibiotics that the patient is receiving or has received in the past 48 hours.
Stability: Serum and body fluid stable 2 hours ambient, 24 hours refrigerated, or 7 days frozen.
Organism stable 7 days ambient. Unstable refrigerated or frozen.
Standard Volume: 2 mL serum or body fluid.
Minimum Volume: 1 mL serum or body fluid.
Transport: Serum or body fluid refrigerated, or frozen on dry ice. Organism ambient on agar slant, packaged according to Infectious Substances Shipping Guidelines.
Comments: As a general rule, peak levels are collected 30 minutes after completion of infusion of an intravenous dose of antimicrobial agent and one hour after an intramuscular or oral dose. Trough levels are collected just prior to the administration of the next dose. If more than one antibiotic is being used, the peak sample should be drawn after completion of the second antibiotic.

The data to support the use of bactericidal assays in the management of complicated bacterial infections such as endocarditis and osteomyelitis are limited. The results of the assay should be interpreted with caution.

Rejection Criteria: Serum or body fluid exceeding stability; non-viable organism; mixed culture; or inadequate information.

PeaceHealth Laboratories