Print View Attached Docs:New method increases sensitivity for Heparin-Induced Thrombocytopenia (HIT) (9/06)
Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 6/11/2010
Performed: Daily
Released: Next day. Submit to laboratory by 10am for same-day testing.
CPT Code: 86022
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST) or one 5 mL red top tube. Also acceptable: One 4.5 mL light blue top tube (sodium citrate). Collect citrate specimen by careful venipuncture to avoid tissue fluid contamination or hemolysis. Do not use needles smaller than 23 gauge and fill tube to maximum draw, at least 90% full. Mix by gentle inversion.
Handling: Centrifuge and remove serum or plasma from cells as soon as possible. Serum or plasma may be stored at 2-8° C if tested within 48 hours. Freeze if serum or plasma will not be assayed within 48 hours. Label with patient name and number (date of birth, barcode,or similar) and “serum” or “plasma,” as appropriate. If multiple tests are ordered, freeze a separate aliquot for this test.
Stability: 4 hours ambient, 48 hours refrigerated, or 7 days frozen.
Standard Volume: 1 mL serum or plasma
Minimum Volume: 200 µL serum or plasma
Transport: Refrigerated, or frozen on dry ice.
Comments: Patient should be off IV heparin for at least 12 hours prior to collection. Confer with physician before discontinuing heparin. Although it may decrease sensitivity of the assay, testing will be performed on patients undergoing heparin therapy upon physician request.
Rejection Criteria: Moderate to marked hemolysis; refrigerated specimen received in laboratory more than 48 hours after collection.

Reference Range:
Negative: HIT result is reported as Negative, Weakly Positive, Positive, and Strongly Positive, with additional guidelines for test interpretation.

PeaceHealth Laboratories