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Alias Names: 5-HIAA / Serotonin Metabolite, Urine
Methodology: High Performance Liquid Chromatography (HPLC)
Edit Date: 6/11/2010
Performed: Sunday, Tuesday-Saturday
Released: 24-72 hours after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 83497-90
Specimen Collection Details
Collection: 24-hour or random urine. Refrigerate during collection, storage and transport. Patients should abstain, if possible, from medications, over-the-counter drugs, and herbal remedies for at least 72 hours prior to the test. 24-hour collection is generally recommended, but random collection may be used. Refrigeration is the most important aspect of specimen preservation.
Handling: 5 mL aliquot from a well-mixed urine collection. Record total volume and collection time interval on transport tube and requisition. Refrigerate.
Stability: 7 days refrigerated or 14 days frozen. Specimen unstable ambient.
Standard Volume: 5 mL urine.
Minimum Volume: 1 mL urine.
Transport: Refrigerated.
Comments: 5-Hydroxyindoleacetic acid (5-HIAA) results are expressed as a ratio to creatinine excretion (mg/ g crt). HIAA mass per day (mg/d) is not reported if the urine collection is random, other than a 24-hour, or for a urine volume less than 400 mL/d.

Foods and medications associated with altered urinary HIAA results:

Decreased HIAA: Aspirin, chlorpromazine (Thorazine), corticotropin, dihydroxyphenylacetic acid, ethanol, gentisic acid, homogentisic acid, hydrazine derivatives, imipramine (Tofranil®), isocarboxazid (Marplan), keto acids, levodopa, MAO inhibitors, methenamine, methyldopa (Aldometperchlorperazine, phenothiazines (Compazine®), promazine, promethazine (Mepergan®).

Increased HIAA: Acetaminophen, acetanilide, caffeine, coumaric acid, diazepam (Valium®), ephedrine, fluorouracil, glycerol guaiacolate (Guaifenesin), melphalan (Alkeran®), mephenesin, methamphetamine (Desoxyn), methocarbamol (Robaxin®), naproxen, nicotine, phenacetin, phenmetrazine, phenobarbital, phentolamine, rauwolfia, reserpine.

Foods: Avocados, bananas, eggplant, pineapple, plums, tomatoes, walnuts.

Foods rich in serotonin and medications which may affect metabolism of serotonin must be avoided at least 72 hours before and during collection of urine for HIAA.

Reference Inteval
Components Reference Interval
5-HIAA, Urine 0.0-15.0 mg/d
5-HIAA, Urine
0-14 mg/g crt
The HIAA-to-creatinine ration
will be reported whenever the urine
collection is random
or other than 24-hour,
or the urine volume is less than
400 mL/24 hours.
Creatinine (24-hour) Male Female
3-8 years 140-700 mg/d 140-700 mg/d
9-12 years 300-1300 mg/d 300-1300 mg/d
13-17 years 500-2300 mg/d 400-1600 mg/d
18-50 years 1000-2500 mg/d 700-1600 mg/d
51-80 years 800-2100 mg/d 500-1400 mg/d
81 years and older 600-2000 mg/d 400-1300 mg/d

PeaceHealth Laboratories