Total semen sample by masturbation into a clean glass or plastic container.
Handling:
Send specimen ambient or refrigerated to lab within 24 hours. If delay is unavoidable, semen may be spread on 2 totally frosted glass slides and submit immersed in PAP fixative.
Stability:
24 hours ambient or refrigerated. Unstable frozen.
Standard Volume:
1 mL semen or 2 slides in PAP fixative.
Minimum Volume:
0.5 mL semen or 1 slide in PAP fixative.
Transport:
Ambient or refrigerated within 24 hours.
Comments:
Please note sperm count on label. Low counts, below 10 M/mL, will require preparation of centrifuged semen slides.
Rejection Criteria:
Frozen semen, insufficient volume.
Reference Range:
>14% normal sperm. WHO strict morphologic criteria used.
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