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89315:BORDETELLA PERTUSSIS ANTIBODIES, IgG, IgM, and IgA | |||||||||
Alias Names: | Whooping Cough | ||||||||
Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
Edit Date: | 6/11/2010 | ||||||||
Performed: | Tuesday | ||||||||
Released: | 2-8 days after setup at PeaceHealth Laboratories’ reference lab | ||||||||
CPT Code: | 86615-90 x3 | ||||||||
Specimen Collection Details | |||||||||
Collection: | One 7.5 mL serum separator tube (SST). | ||||||||
Handling: | Allow to clot centrifuge and immediately separate serum from cells into a plastic vial. Refrigerate. | ||||||||
Stability: | 48 hours ambient, 14 days refrigerated, or 1 year frozen | ||||||||
Standard Volume: | 1 mL serum | ||||||||
Minimum Volume: | 300 µL serum | ||||||||
Transport: | Refrigerated. | ||||||||
Comments: | Detectable levels of IgG antibodies to B. pertussis may be seen in the serum of vaccinated individuals of all ages, in early infancy as the result of placental transfer, and in the convalescent phase of a recent infection. IgG antibodies can only be used to diagnose active infection when paired sera are available and a rise in antibody level can be demonstrated. A significant rise may not always be demonstrated as peak levels of IgG may be reached before the first specimen is collected. Therefore, IgA and IgM antibody levels should be measured to diagnose active diseases. | ||||||||
Rejection Criteria: | Severely lipemic, contaminated, heat-inactivated, or hemolyzed specimen. |
Reference Range: | |||||||||||||||||||
COMPONENTS | REFERENCE INTERVAL | ||||||||||||||||||
Bordetella pertussis Antibody, IgA
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≤0.9 U/mL
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Negative – No significant level of detectable Bordetella pertussis IgA antibody.
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1.0-1.1 U/mL
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Equivocal – Repeat testing in 10-14 days may be helpful.
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≥1.2 U/mL
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Positive – IgA antibody to Bordetella pertussis detected which may indicate a current or past exposure/immunization to B. pertussis.
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Bordetella pertussis Antibody, IgG
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≤0.9 U/mL
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Negative – No significant level of detectable Bordetella pertussis IgG antibody.
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1.0-2.4 U/mL
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Equivocal – Repeat testing in 10-14 days may be helpful.
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≥ 2.5 U/mL
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Positive – IgG antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.
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Bordetella pertussis Antibody, IgM
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≤0.9 U/mL
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Negative – No significant level of detectable Bordetella pertussis IgM antibody.
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1.0-1.1 U/mL
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Equivocal – Repeat testing in 10-14 days may be helpful.
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≥1.2 U/mL
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Positive – IgM antibody to Bordetella pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
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