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Methodology: Chemiluminescent Immunoassay (CLIA)
Edit Date: 6/25/2009
Performed: Daily
Released: Same day
CPT Code: 80400
Specimen Collection Details
Collection: Two specimens total: One 7.5 mL serum separator tube (SST) per draw. Also acceptable: One 4.5 mL light green top plasma separator tube (PST), one 5 mL red top tube, one 4 mL lavender top tube, or one 4 mL green top tube (heparin) per draw.
Physician will recommend appropriate time to draw specimens.
Handling: Allow to clot, centrifuge and separate serum or plasma within 2 hours. Refrigerate. Note collection time and number in series, e.g., “1 of 2,” etc., on each specimen. Freeze at -20° C if specimen cannot be assayed within 48 hours. Allow no more than one freeze/thaw cycle.
Stability: 48 hours refrigerated.
Standard Volume: 0.5 mL serum per draw.
Minimum Volume: 100 µL serum per draw.
Transport: Refrigerated, or frozen on dry ice.
Comments: The measurement of serum cortisol, utilizing morning and evening levels, aids in the diagnosis of adrenal-related disease.

See also: ACTH Stimulation Test (40381)

Rejection Criteria: Specimen frozen and thawed more than once.

Refrence Range:
AM 6-23 µg/dL
PM 2-12 µg/dL

PeaceHealth Laboratories