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| 40383:CORTISOL, 2 SPECIMENS | |||||||||
| Methodology: | Chemiluminescent Immunoassay (CLIA) | ||||||||
| Edit Date: | 6/25/2009 | ||||||||
| Performed: | Daily | ||||||||
| Released: | Same day | ||||||||
| CPT Code: | 80400 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | Two specimens total: One 7.5 mL serum separator tube (SST) per draw. Also acceptable: One 4.5 mL light green top plasma separator tube (PST), one 5 mL red top tube, one 4 mL lavender top tube, or one 4 mL green top tube (heparin) per draw. Physician will recommend appropriate time to draw specimens. |
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| Handling: | Allow to clot, centrifuge and separate serum or plasma within 2 hours. Refrigerate. Note collection time and number in series, e.g., “1 of 2,” etc., on each specimen. Freeze at -20° C if specimen cannot be assayed within 48 hours. Allow no more than one freeze/thaw cycle. | ||||||||
| Stability: | 48 hours refrigerated. | ||||||||
| Standard Volume: | 0.5 mL serum per draw. | ||||||||
| Minimum Volume: | 100 µL serum per draw. | ||||||||
| Transport: | Refrigerated, or frozen on dry ice. | ||||||||
| Comments: | The measurement of serum cortisol, utilizing morning and evening levels, aids in the diagnosis of adrenal-related disease.
See also: ACTH Stimulation Test (40381) |
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| Rejection Criteria: | Specimen frozen and thawed more than once. | ||||||||
| Refrence Range: | |||||||||||||||||||
| AM 6-23 µg/dL PM 2-12 µg/dL |
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