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| 40361:CORTISOL, 3 SPECIMENS | |||||||||
| Methodology: | Chemiluminescent Immunoassay (CLIA) | ||||||||
| Edit Date: | 6/25/2009 | ||||||||
| Performed: | Daily | ||||||||
| Released: | Same day | ||||||||
| CPT Code: | 80400 / 82533 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | Three specimens total: One 7.5 mL serum separator tube (SST) per draw. Also acceptable: One 4.5 mL light green top plasma separator tube (PST), one 5 mL red top tube, or one 4 mL green top tube (heparin) per draw. Physician will recommend appropriate time to draw specimens. |
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| Handling: | Allow to clot, centrifuge and separate serum from cells within 2 hours. Refrigerate. Note collection time and number in series, e.g., “1 of 3,” etc., on each specimen. Freeze at -20° C or colder if specimen cannot be assayed within 48 hours. Allow no more than one freeze/thaw cycle. | ||||||||
| Stability: | 48 hours refrigerated. | ||||||||
| Standard Volume: | 0.5 mL serum per draw. | ||||||||
| Minimum Volume: | 100 µL serum per draw. | ||||||||
| Transport: | Refrigerated, or frozen on dry ice. | ||||||||
| Comments: | If this is an ACTH Stimulation test, use Cortisol Stimulation Test ACTH (40381) . | ||||||||
| Rejection Criteria: | Specimens frozen and thawed more than once. | ||||||||
| Reference Range: | |||||||||||||||||||
| AM 6-23 µg/dL PM 2-12 µg/dL |
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