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Methodology: Chemiluminescent Immunoassay (CLIA)
Edit Date: 6/25/2009
Performed: Daily
Released: Same day
CPT Code: 80400 / 82533
Specimen Collection Details
Collection: Three specimens total: One 7.5 mL serum separator tube (SST) per draw. Also acceptable: One 4.5 mL light green top plasma separator tube (PST), one 5 mL red top tube, or one 4 mL green top tube (heparin) per draw.
Physician will recommend appropriate time to draw specimens.
Handling: Allow to clot, centrifuge and separate serum from cells within 2 hours. Refrigerate. Note collection time and number in series, e.g., “1 of 3,” etc., on each specimen. Freeze at -20° C or colder if specimen cannot be assayed within 48 hours. Allow no more than one freeze/thaw cycle.
Stability: 48 hours refrigerated.
Standard Volume: 0.5 mL serum per draw.
Minimum Volume: 100 µL serum per draw.
Transport: Refrigerated, or frozen on dry ice.
Comments: If this is an ACTH Stimulation test, use Cortisol Stimulation Test ACTH (40381) .
Rejection Criteria: Specimens frozen and thawed more than once.

Reference Range:
AM 6-23 µg/dL
PM 2-12 µg/dL

PeaceHealth Laboratories