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Methodology: Mechanical Clot Detection following enzymatic neutralization of heparin
Edit Date: 9/19/2008
Performed: Daily
Released: Same day
CPT Code: 85525
Specimen Collection Details
Collection: One 4.5 mL or three 2.7 mL light blue top tubes (sodium citrate). Collect by careful venipuncture to avoid tissue contamination or hemolysis. Do not use needles smaller than 23 gauge. Fill tube to maximum draw (at least 90% full). Mix by gentle inversion.
Handling: Due to short stability, specimen must either be sent STAT to arrive at the laboratory testing department within 4 hours, or sent frozen. For STAT submit whole blood in original unopened tube. Call for STAT pick up. For standard transport, centrifuge to produce platelet poor plasma (at least 1700 X G for 15 minutes) and using a plastic pipette, transfer the top 2/3 of the plasma into a plastic vial. Label with patient name, I.D. number, test and “citrated plasma.” and freeze immediately. Send to the laboratory within 7 days. If multiple tests are ordered, freeze a separate aliquot for this test.
Stability: 4 hours refrigerated, or 7 days frozen.
Standard Volume: 5 mL whole blood or 2 mL plasma.
Minimum Volume: 3 mL whole blood or 0.5 mL plasma.
Transport: Original unopened tube refrigerated if sent STAT, or citrated plasma frozen on dry ice in a Styrofoam container.
Comments: A specific heparinase enzyme is used in this assay. Standard heparin will be neutralized up to a concentration of 2.0 U/mL.
Rejection Criteria: Serum; visible fibrin; moderate to marked hemolysis; specimen received in testing department more than 4 hours after collection.

PeaceHealth Laboratories