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| 69150:ANTINUCLEAR ANTIBODIES (ANA) TITER | |||||||||
| Methodology: | Indirect Fluorescent Antibody (IFA) | ||||||||
| Edit Date: | 4/19/2010 | ||||||||
| Performed: | Monday- Friday | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 86039 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | NOTE: Do not order this test unless the ANA screen was performed off-site and ONLY an ANA Titer is wanted. One 7.5 mL serum separator tube (SST). |
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| Handling: | Allow SST to clot 10 minutes; centrifuge, separate serum into a plastic vial. Refrigerate until transport. Freeze serum if it is to be held longer than 5 days before testing. | ||||||||
| Standard Volume: | 1 mL serum. | ||||||||
| Minimum Volume: | 100 µL serum. | ||||||||
| Transport: | Refrigerated. | ||||||||
| Comments: | ANA samples are screened using an EIA assay. See Antinuclear Antibodies (ANA) Screen (69100) . All samples that screen positive or indeterminate are titered using HEp-2 cells. The titer and pattern will be reported. | ||||||||
| Rejection Criteria: | Plasma, gross lipemia, hemolysis, or contaminated specimen. | ||||||||