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|69150:ANTINUCLEAR ANTIBODIES (ANA) TITER|
|Methodology:||Indirect Fluorescent Antibody (IFA)|
|Released:||Same day as tested|
|Specimen Collection Details|
|Collection:||NOTE: Do not order this test unless the ANA screen was performed off-site and ONLY an ANA Titer is wanted.
One 7.5 mL serum separator tube (SST).
|Handling:||Allow SST to clot 10 minutes; centrifuge, separate serum into a plastic vial. Refrigerate until transport. Freeze serum if it is to be held longer than 5 days before testing.|
|Standard Volume:||1 mL serum.|
|Minimum Volume:||100 µL serum.|
|Comments:||ANA samples are screened using an EIA assay. See Antinuclear Antibodies (ANA) Screen (69100) . All samples that screen positive or indeterminate are titered using HEp-2 cells. The titer and pattern will be reported.|
|Rejection Criteria:||Plasma, gross lipemia, hemolysis, or contaminated specimen.|