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| 80215:WEST NILE VIRUS ANTIBODY, IgG | |||||||||
| Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
| Edit Date: | 11/12/2008 | ||||||||
| Performed: | Tuesday, Friday | ||||||||
| Released: | 1-5 days after setup at PeaceHealth Laboratories’ reference lab | ||||||||
| CPT Code: | 86789-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). | ||||||||
| Handling: | Allow to clot, centrifuge and separate serum from cells immediately and pour into a plastic vial. Refrigerate. | ||||||||
| Stability: | 48 hours ambient, 14 days refrigerated, or 1 year frozen. Allow no more than one freeze/thaw cycle. | ||||||||
| Standard Volume: | 1 mL serum | ||||||||
| Minimum Volume: | 0.5 mL serum | ||||||||
| Transport: | Refrigerated. | ||||||||
| Comments: | This test is intended to be used as a semi-quantitative means of detecting West Nile virus-specific IgG in serum specimens in which there is a clinical suspicion of West Nile virus infection. | ||||||||
| Rejection Criteria: | Plasma; severely lipemic, contaminated, heat-activated, or hemolyzed specimen. | ||||||||
| Referene Range: | |||||||||||||||||||
| ≤ 1.29 IV |
Negative – No significant level of West Nile virus IgG antibody detected.
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| 1.30-1.50 IV |
Equivocal – Questionable presence of West Nile virus IgG antibody detected. Repeat testing in 10-14 days may be helpful.
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| ≥ 1.51 IV |
Positive – Presence of IgG antibody to West Nile virus detected, suggestive of current or past infection.
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| Seroconversion between acute and convalescent sera is considered strong evidence of current or recent infection. The best evidence for infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time. | |||||||||||||||||||