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Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 11/12/2008
Performed: Tuesday, Friday
Released: 1-5 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 86789-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and separate serum from cells immediately and pour into a plastic vial. Refrigerate.
Stability: 48 hours ambient, 14 days refrigerated, or 1 year frozen. Allow no more than one freeze/thaw cycle.
Standard Volume: 1 mL serum
Minimum Volume: 0.5 mL serum
Transport: Refrigerated.
Comments: This test is intended to be used as a semi-quantitative means of detecting West Nile virus-specific IgG in serum specimens in which there is a clinical suspicion of West Nile virus infection.
Rejection Criteria: Plasma; severely lipemic, contaminated, heat-activated, or hemolyzed specimen.

Referene Range:
≤ 1.29 IV
Negative – No significant level of West Nile virus IgG antibody detected.
1.30-1.50 IV
Equivocal – Questionable presence of West Nile virus IgG antibody detected. Repeat testing in 10-14 days may be helpful.
≥ 1.51 IV
Positive – Presence of IgG antibody to West Nile virus detected, suggestive of current or past infection.
Seroconversion between acute and convalescent sera is considered strong evidence of current or recent infection. The best evidence for infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

PeaceHealth Laboratories