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Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 8/16/2010
Performed: Monday, Thursday
Released: 1-5 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 86682-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Centrifuge and separate serum from cells immediately. Label specimen clearly as ‘acute’ or ‘convalescent’. Refrigerate.
Stability: 4 hours ambient, 72 hours refrigerated, or 1 year frozen (avoid repeated freeze/thaw cycles).
Standard Volume: 1 mL serum.
Minimum Volume: 150 µL serum.
Transport: Refrigerated.
Comments: Parallel testing is preferred and the convalescent specimen must be received within 30 days from receipt of the acute specimen. Please mark specimens plainly as ‘acute’ or ‘convalescent.’
Rejection Criteria: Plasma; severe lipemia; contaminated specimen.

Reference Range:
≤ 0.235 OD
Negative – No significant level of Echinococcus IgG antibody detected.
0.236-0.299 OD
Equivocal – Questionable presence of Echinococcus IgG antibody detected. Repeat testing in 10-14 days may be helpful.
≥ 0.300 OD
Positive – Presence of IgG antibody to Echinococcus detected, suggestive of current or past infection.
Patients with collagen vascular diseases, hepatic cirrhosis, schistosomiasis, and other parasitic infections can produce false-positive results. There is a strong cross-reaction between echinococcosis and cysticercosis positive sera.

Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time

PeaceHealth Laboratories