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| 83420:ECHINOCOCCUS ANTIBODY, IgG, SERUM | |||||||||
| Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
| Edit Date: | 8/16/2010 | ||||||||
| Performed: | Monday, Thursday | ||||||||
| Released: | 1-5 days after setup at PeaceHealth Laboratories’ reference lab | ||||||||
| CPT Code: | 86682-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). | ||||||||
| Handling: | Centrifuge and separate serum from cells immediately. Label specimen clearly as ‘acute’ or ‘convalescent’. Refrigerate. | ||||||||
| Stability: | 4 hours ambient, 72 hours refrigerated, or 1 year frozen (avoid repeated freeze/thaw cycles). | ||||||||
| Standard Volume: | 1 mL serum. | ||||||||
| Minimum Volume: | 150 µL serum. | ||||||||
| Transport: | Refrigerated. | ||||||||
| Comments: | Parallel testing is preferred and the convalescent specimen must be received within 30 days from receipt of the acute specimen. Please mark specimens plainly as ‘acute’ or ‘convalescent.’ | ||||||||
| Rejection Criteria: | Plasma; severe lipemia; contaminated specimen. | ||||||||
| Reference Range: | |||||||||||||||||||
| ≤ 0.235 OD |
Negative – No significant level of Echinococcus IgG antibody detected.
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| 0.236-0.299 OD |
Equivocal – Questionable presence of Echinococcus IgG antibody detected. Repeat testing in 10-14 days may be helpful.
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| ≥ 0.300 OD |
Positive – Presence of IgG antibody to Echinococcus detected, suggestive of current or past infection.
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| Patients with collagen vascular diseases, hepatic cirrhosis, schistosomiasis, and other parasitic infections can produce false-positive results. There is a strong cross-reaction between echinococcosis and cysticercosis positive sera.
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time |
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