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Methodology: Direct Fluorescent Antibody (DFA)
Edit Date: 3/25/2010
Performed: Daily
Released: 24-48 hours after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 87260-90
Specimen Collection Details
Collection: Submit 2 acetone-fixed slides or specimens in viral transport media consisting of nasal swab or washings, eye swab, rectal swab, or 1-2 grams fresh unpreserved stool in sterile container.
Handling: Source of specimen is requested.
Stability: Acetone-fixed slides: 48 hours ambient, 72 hours refrigerated, or 7 days frozen.
M4 Transport Media: 24 hours ambient or 48 hours refrigerated.
Fresh stool: 24 hours ambient, 72 hours refrigerated, or 7 days frozen.
Transport: Acetone-fixed slides: Ambient
M4 Transport Media/Swab: Refrigerated
Fresh stool: Refrigerated
Comments: Specimen should be collected early in the acute phase of infection. The chance of viral recovery is best during the first 3 days after onset and with many viruses is greatly reduced beyond 5 days.
Rejection Criteria: Wooden or dry swabs; calcium alginate; specimens frozen at -20° C, as virus loses infectivity.

PeaceHealth Laboratories