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Alias Names: Treponema pallidum Antibody / FTA Abs / IgG by IFA / Syphilis
Methodology: Indirect Fluorescent Antibody (IFA)
Edit Date: 1/18/2010
Performed: Daily
Released: 1-4 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 86780-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Centrifuge and separate serum into a plastic vial immediately.
Stability: 24 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/that cycles.
Standard Volume: 1 mL serum.
Minimum Volume: 0.5 mL serum.
Transport: Refrigerated.
Comments: The Fluorescent Treponema Antibody (FTA) is recommended for follow-up of reactive nontreponemal tests for syphilis, and as a single test in patients suspected of late syphilis. The FTA may be used to resolve discrepancies between laboratory results and clinical impressions. FTA tests for syphilis may be falsely positive in some cases of systemic lupus erythematosus, pregnancy, and leprosy. The FTA test is not recommended for cerebrospinal fluid specimens. For CSF specimens, the VDRL-CSF test is recommended. Inconclusive results will be repeated by TP-PA to aid the physician in diagnosis. Inconclusive final reports indicate the initial specimen submitted has been tested twice and cannot be interpreted as either reactive or nonreactive. If it is the second specimen submitted on a patient and the report is again inconclusive, it is impossible to state definitively that the patient does or does not have syphilitic infection.

Reflex will only happen when the FTA is found to be inconclusive; if reflexed, add additional 86780.

Rejection Criteria: CSF, plasma, or other body fluids; severely lipemic, contaminated, or hemolyzed specimen.

PeaceHealth Laboratories