Print View Attached Docs:
Alias Names: EBV
Methodology: Real-Time Polymerase Chain Reaction (RT-PCR)
Edit Date: 6/17/2010
Performed: Daily
Released: 1-4 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 87799-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 4 mL lavender top tube or cerebral spinal fluid (CSF).
Handling: Allow to clot, centrifuge and separate serum or plasma into a plastic vial. Freeze. Indicate source on label.
Stability: 8 hours ambient, 72 hours refrigerated, or 1 year frozen.
Standard Volume: 1 mL plasma, serum, or CSF.
Minimum Volume: 250 µL plasma, serum, or CSF.
Transport: Frozen on dry ice.
Rejection Criteria: Whole blood; heparinized specimen.

Reference Range:
The limit of quantification for this DNA assay is 2.6 log copies/mL (390 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as “<2.6 log copies/mL (<390 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified." The quantitative range of this assay is 2.6-7.6 log copies/mL (390-39,000,000 copies/mL). A negative result (less than 2.6 log copies/mL or less than 390 copies/mL) does not rule out the presence of PCR inhibitors in the patient specimen, or EBV DNA nucleic acid in concentrations below the level of detection of the assay. Inhibition may also lead to underestimation of viral quantitation. No international standard is currently available for calibration of this assay. Caution should be taken when interpreting results generated by different assay methodologies.

PeaceHealth Laboratories