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| 96310:ABL SEQUENCING | |||||||||
| Alias Names: | ABL Mutation Screening / BCR-ABL Sequencing / Imatinib (Gleevec) Resistance / TK Receptor | ||||||||
| Methodology: | Sequencing | ||||||||
| Edit Date: | 4/13/2009 | ||||||||
| Performed: | Once weekly | ||||||||
| Released: | 7-10 days after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 83891-902B / 83894-902B / 83898-902B x2 / 83909-902B x2 / 83912-902B / 86902-902B | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | Six to seven 4 mL lavender top tube (EDTA) peripheral blood. Draw Monday-Thursday only. Also acceptable: Bone marrow. Note that the lower volume of bone marrow specimen reduces the number of cells and therefore the probability of detecting very low-level leukemia burdens in patients with minimal residual disease. media. | ||||||||
| Handling: | BLOOD: Stabilize peripheral blood by mixing with RPMI media in equal volumes of blood to media (1:1 ratio). Keep refrigerated. Indicate on the requisition the volume of RPMI tissue culture media used. Specimen MUST be received at PeaceHealth Laboratories’ reference lab within 24 hours of collection. BONE MARROW: Draw in a heparinized syringe and transfer to an EDTA collection tube. Mix with an equal volume (1:1 ratio) of RPMI. Keep refrigerated. |
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| Stability: | Unstable frozen. | ||||||||
| Standard Volume: | 30 mL whole blood. | ||||||||
| Minimum Volume: | 20 mL whole blood. | ||||||||
| Transport: | Refrigerated. | ||||||||
| Comments: | Direct sequencing of the ABL kinase domain. | ||||||||
| Rejection Criteria: | Clotted, frozen, or leaking specimen.
This test may require insurance company prior authorization before ordering.
Please check the prior authorization list . Failure to gain preauthorization may result in denial of coverage. |
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