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Methodology: Chemiluminescence (CL)
Edit Date: 6/25/2009
Performed: Monday-Friday
Released: Next day
CPT Code: 80400 / 82533 x3
Specimen Collection Details
Collection: Five specimens total: One 7 mL serum separator tube (SST) per draw. Physician will recommend appropriate time to draw specimens.
Handling: Allow to clot, centrifuge and separate serum from cells within 2 hours. Refrigerate. Note collection time and number in series, e.g., “3 of 5,” etc., on each specimen. Freeze at -20° C if specimen will not be assayed within 48 hours. Allow no more than one freeze/thaw cycle. Store ambient no more than 8 hours.
Stability: 48 hours refrigerated.
Standard Volume: 0.5 mL serum per draw.
Minimum Volume: 100 µL serum per draw.
Transport: Refrigerated, or frozen on dry ice.
Comments: Cortisol is the major glucocoticoid produced and secreted by the adrenal cortex. The measurement of serum cortisol, utilizing morning and evening levels, aids in the diagnosis of adrenal-related disease.
Rejection Criteria: Gross hemolysis or lipemia; specimens frozen and thawed more than once.

Reference Range:
AM: 6-23 µg/dL
PM: 2-12 µg/dL

PeaceHealth Laboratories