High Performance Liquid Chromatography / Tandem Mass Spectrometry (HPLC/MS/MS)
Edit Date:
11/17/2010
Performed:
Daily
Released:
1-4 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code:
84403-90
Specimen Collection Details
Collection:
One 7.5 mL serum separator tube (SST). Also acceptable: One 4 mL green top tube (heparin). NOTE: Specimen should be collected between 6 a.m. and 10 a.m.
Handling:
Allow to clot, centrifuge and immediately separate serum or plasma into a plastic vial. Refrigerate.
Stability:
24 hours ambient, 7 days refrigerated, or 6 months frozen.
Standard Volume:
1 mL serum or plasma
Minimum Volume:
200 µL serum or plasma
Transport:
Refrigerated.
Comments:
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.
Rejection Criteria:
EDTA plasma.
Reference Range:
Female
Male
Premature (26-28 weeks)
5-16 ng/dL
Premature (26-28 weeks)
59-125 ng/dL
Premature (31-35 weeks)
5-22 ng/dL
Premature (31-35 weeks)
37-198 ng/dL
Newborn
20-64 ng/dL
Newborn
75-400 ng/dL
1-7 months
Levels decrease during the first month to less than 10 ng/dL and remain at this level until puberty.
1-7 months
Levels decrease rapidly the first week to 20-50 ng/dL, and then increase to 60-400 ng/dL between 20-60 days. Levels then decline to pre-pubertal range levels of 3-10 ng/dL by seven months.