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| 33362:PHENYTOIN (DILANTIN), FREE and TOTAL | |||||||||
| Methodology: | Fluorescent Polarization Immunoassay (FPIA) | ||||||||
| Edit Date: | 8/24/2009 | ||||||||
| Performed: | Daily | ||||||||
| Released: | Same day as tested | ||||||||
| CPT Code: | 80185 / 80186 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 5 mL red top tube. Do not use serum separator tube (SST) or gels. | ||||||||
| Handling: | Allow to clot, centrifuge and immediately separate serum from cells. Refrigerate. Freeze if testing is delayed more than 24 hours. | ||||||||
| Stability: | 24 hours refrigerated or 3 months frozen. Unstable ambient. | ||||||||
| Standard Volume: | 2 mL serum | ||||||||
| Minimum Volume: | 1 mL serum | ||||||||
| Transport: | Refrigerated, or frozen on dry ice. | ||||||||
| Comments: | Free Phenytoin is not available without a Total Phenytoin Value. Specimen from a patient receiving fosphenytoin should be drawn at least 2 hours following IV administration and 4 hours following IM administration. Date and time of last dose is requested. | ||||||||
| Rejection Criteria: | Use of serum separator tube (SST) or gels. | ||||||||
| Reference Range: | |||||||||||||||||||
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Free Phenytoin
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1.0-2.0 µg/mL
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Percent Free Phenytoin
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8.0-14.0%
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Total Phenytoin
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10.0-20.0 µg/mL
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