33362:PHENYTOIN (DILANTIN), FREE and TOTAL

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33362:PHENYTOIN (DILANTIN), FREE and TOTAL
Methodology: Fluorescent Polarization Immunoassay (FPIA)
Edit Date: 8/24/2009
Performed: Daily
Released: Same day as tested
CPT Code: 80185 / 80186
Specimen Collection Details
Collection: One 5 mL red top tube. Do not use serum separator tube (SST) or gels.
Handling: Allow to clot, centrifuge and immediately separate serum from cells. Refrigerate. Freeze if testing is delayed more than 24 hours.
Stability: 24 hours refrigerated or 3 months frozen. Unstable ambient.
Standard Volume: 2 mL serum
Minimum Volume: 1 mL serum
Transport: Refrigerated, or frozen on dry ice.
Comments: Free Phenytoin is not available without a Total Phenytoin Value. Specimen from a patient receiving fosphenytoin should be drawn at least 2 hours following IV administration and 4 hours following IM administration. Date and time of last dose is requested.
Rejection Criteria: Use of serum separator tube (SST) or gels.

Reference Range:
Free Phenytoin
1.0-2.0 µg/mL
Percent Free Phenytoin
8.0-14.0%
Total Phenytoin
10.0-20.0 µg/mL