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Methodology: High Performance Liquid Chromatography/Tandem Mass Spectrometry (HPLC/MS/MS)
Edit Date: 11/17/2010
Performed: Daily
Released: 1-4 days after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 84402-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 4 mL green top tube (heparin). Specimen should be collected between 6 a.m. and 10 a.m.
Handling: Centrifuge and separate serum or plasma from cells and immediately pour into a plastic vial. Refrigerate.
Stability: 24 hours ambient, 7 days refrigerated, or 6 months frozen.
Standard Volume: 1 mL serum or plasma.
Minimum Volume: 0.4 mL serum or plasma.
Transport: Refrigerated.
Comments: This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS. Testosterone Free, Females and Children by tandem mass spectrometry, only provides a result for the free testosterone value (pg/mL).
Rejection Criteria: EDTA plasma.

Reference Ranges:
Female Free Testosterone Male Free Testosterone
1-6 years <0.6 pg/mL 1-6 years <0.6 pg/mL
7-9 years 0.6-1.8 pg/mL 7-9 years 0.1-0.9 pg/mL
10-11 years 0.1-3.5 pg/mL 10-11 years 0.1-6.3 pg/mL
12-13 years 0.9-6.8 pg/mL 12-13 years 0.5-98.0 pg/mL
14-15 years 1.2-7.5 pg/mL 14-15 years 3.0-138.0 pg/mL
16-17 years 1.2-9.9 pg/mL 16-17 years 38.0-173.0 pg/mL
18-30 years 0.8-7.4 pg/mL 18 years and older 47.0-244.0 pg/mL
31-40 years 1.3-9.2 pg/mL Tanner Stage I ≤ 3.7 pg/mL
41-51 years 1.1-5.8 pg/mL Tanner Stage II 0.3-21.0 pg/mL
Postmenopausal 0.6-3.8 pg/mL Tanner Stage III 1.0-98.0 pg/mL
Tanner Stage I <2.2 pg/mL Tanner Stage IV 35.0-169.0 pg/mL
Tanner Stage II 0.4-4.5 pg/mL Tanner Stage V 41.0-239.0 pg/mL
Tanner Stage III 1.3-7.5 pg/mL
Tanner Stage IV 1.1-15.5 pg/mL
Tanner Stage V 0.8-9.2 pg/mL

PeaceHealth Laboratories