 Print View Attached Docs: |
|||||||||
| 58500:SWINE-ORIGIN INFLUENZA A/H1N1 (2009) DUPLEX by RT-PCR | |||||||||
| Alias Names: | Flu A/Swine H1N1 / SWFLU RT PCR / /SOIA Detect / INFA / AOIA | ||||||||
| Methodology: | RT-PCR | ||||||||
| Edit Date: | 5/15/2010 | ||||||||
| Performed: | Daily from 1 October – 30 April Mondays from 1 May – 31 September |
||||||||
| Released: | 24-48 hours | ||||||||
| CPT Code: | 87798 x2 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | Using a sterile polyester flexible shaft nasopharyngeal swab, collect a nasopharyngeal specimen. Place the swab in M4/M4RT transport media. Also acceptable: Use of a sterile polyester flexible shaft nasopharyngeal swab in combination with a sterile polyester nasal swab(s). Collect a nasopharyngeal and a nasal specimen(s). Place the swab(s) in M4/M4RT transport media. | ||||||||
| Handling: | Refrigerate at 2-8° C, or freeze. | ||||||||
| Stability: | 48 hours refrigerated, or 3 months frozen. Unstable ambient. | ||||||||
| Standard Volume: | 3 mL transport medium. | ||||||||
| Minimum Volume: | 400 µL transport medium. | ||||||||
| Transport: | Refrigerated, or frozen on dry ice. | ||||||||
| Comments: | RT-PCR has a higher sensitivity to detect influenza viruses compared to rapid influenza A&B antigen testing. A negative rapid influenza A&B antigen test result does not rule out novel influenza A (H1N1) virus infection.
This test is not intended for the evaluation of anti-viral resistance of influenza A. A positive result from an rt-PCR test meets the CDC definition of a laboratory-confirmed case of novel influenza A H1N1(2009). For formal confirmation testing (i.e. sequencing), clinicians should inquire with their local state health laboratories or the CDC at http://www.cdc.gov/h1n1flu/specimencollection.htm . |
||||||||
| Rejection Criteria: | Sputum; bronchoalveolar lavage (bronch wash); nasal aspirate; throat swab; nasal swab alone; leaking or non-sterile containers. | ||||||||
| Analytical Characteristics | |||||||||||||||||||
| • Sensitivity 99%
• Specificity 98% • Positive Predictive Value 92% • Negative Predictive Value 100% |
|||||||||||||||||||