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Methodology:Complement Fixation (CF)
Edit Date:10/12/2009
Released:2-5 days after setup at PeaceHealth Laboratories' reference lab
CPT Code:86658-90
Specimen Collection Details
Collection:One 7.5 mL serum separator tube (SST).
Handling:Centrifuge and separate serum into a plastic via within one hour of collection.
Stability:48 hours ambient, 14 days refrigerated, or 1 year frozen.
Standard Volume:1 mL serum
Minimum Volume:0.5 mL serum
Comments:Paired testing of acute and convalescent specimens is recommended. Label tubes clearly as 'acute' or 'convalescent.'
Rejection Criteria:Severely lipemic, hemolyzed, or contaminated specimen.
Reference Range:
< 1:8

Single positive antibody titers of greater than 1:32 may indicate past or current infection. Seroconversion or an increase in titers between acute and convalescent sera of at least fourfold is considered strong evidence of current or recent infection.