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Methodology: Complement Fixation (CF)
Edit Date: 10/12/2009
Performed: Monday-Friday
Released: 2-5 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 86658-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Centrifuge and separate serum into a plastic via within one hour of collection.
Stability: 48 hours ambient, 14 days refrigerated, or 1 year frozen.
Standard Volume: 1 mL serum
Minimum Volume: 0.5 mL serum
Transport: Refrigerated.
Comments: Paired testing of acute and convalescent specimens is recommended. Label tubes clearly as ‘acute’ or ‘convalescent.’
Rejection Criteria: Severely lipemic, hemolyzed, or contaminated specimen.

Reference Range:
< 1:8 Single positive antibody titers of greater than 1:32 may indicate past or current infection. Seroconversion or an increase in titers between acute and convalescent sera of at least fourfold is considered strong evidence of current or recent infection.

PeaceHealth Laboratories