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| 86145:GANGLIOSIDE ANTIBODY, IgG and IgM | |||||||||
| Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
| Edit Date: | 11/17/2010 | ||||||||
| Components: | Ganglioside asialo-GM1/ GM1 / GM2 / GD1a / GD1b / GQ1b | ||||||||
| Performed: | Monday, Wednesday, Friday | ||||||||
| Released: | 1-4 days after setup at PeaceHealth Laboratories’ laboratory. | ||||||||
| CPT Code: | 83516-90 x6 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). | ||||||||
| Handling: | Allow to clot, centrifuge and immediately separate serum into a plastic vial. Freeze. CRITICAL FROZEN. | ||||||||
| Stability: | 1 year frozen. Unstable ambient or refrigerated. | ||||||||
| Standard Volume: | 1 mL serum. | ||||||||
| Minimum Volume: | 0.5 mL serum. | ||||||||
| Transport: | Frozen on dry ice. | ||||||||
| Rejection Criteria: | Plasma or other body fluids; severely lipemic, hemolyzed, contaminated, or heat-inactivated specimen. | ||||||||
| Reference Range: | |||||||||||||||||||
| Components | Reference Interval | ||||||||||||||||||
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Ganglioside (asialo-GM1) Antibody, IgG/IgM Ganglioside (GM1) Antibody, IgG/IgM Ganglioside (GM2) Antibody, IgG/IgM Ganglioside (GD1a) Antibody, IgG/IgM Ganglioside (GD1b) Antibody, IgG/IgM Ganglioside (GQ1b) Antibody, IgG/IgM |
≤ 29 IV: Negative
30-50 IV: Equivocal 51-150 IV: Positive ≥ 151 IV: Strong Positive |
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| Ganglioside antibodies are associated with diverse peripheral neuropathies. Elevated antibody levels to ganglioside-monosialic acid (GM1) and the neutral glycolipid, asialo-GM1 are associated with motor or sensorimotor neuropathies, particularly multifocal motor neuropathy. Anti-GM1 may occur as IgM (polyclonal or monoclonal) or IgG antibodies. These antibodies may also be found in patients with diverse connective tissue diseases as well as normal individuals. GD1a antibodies are associated with different variants of Guillain-Barrsyndrome (GBS) particularly acute motor axonal neuropathy while GD1b antibodies are predominantly found in sensory ataxic neuropathy syndrome. Anti-GQ1b antibodies are seen in more than 80% of patients with Miller-Fisher syndrome and may be elevated in GBS patients with ophthalmoplegia. The role of isolated anti-GM2 antibodies is unknown. These tests by themselves are not diagnostic and should be used in conjunction with other clinical parameters to confirm disease. | |||||||||||||||||||