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Methodology: Polymerase Chain Reaction (PCR)
Edit Date: 2/16/2010
Performed: Monday-Friday
Released: 7-10 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 83891-90 / 83894-90 / 83898-90 x2 / 83912-90
Specimen Collection Details
Collection: Fresh frozen tissue, paraffin embedded tissue, or sterile fluid.
Transport: Fresh tissue or sterile fluids frozen. Paraffin block ambient.
Comments: 16S rRNA gene contains conserved regions useful for the design of universal PCR primers that can amplify various fragments of the 16S rRNA gene from all pathogenic and nonpathogenic bacteria. These fragments include hypervariable regions containing species-specific signature sequences useful for bacterial identification to species level.
Rejection Criteria: Citrated or heparinized solutions; respiratory, genitourinary, or direct blood specimen; swab specimen.

PeaceHealth Laboratories