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Methodology: Indirect Fluorescent Antibody
Edit Date: 4/5/2010
Performed: Monday-Friday
Released: 3-5 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 86713-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and immediately separate serum into a plastic vial. Mark specimen plainly as “acute” or “convalescent.”
Stability: 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
Standard Volume: 1 mL serum
Minimum Volume: 0.5 mL serum
Transport: Refrigerated.
Comments: Parallel testing is preferred and convalescent specimen must be received within 30 days of receipt of acute specimen. Acute and convalescent tubes must be marked as such.
Rejection Criteria: Severely lipemic, contaminated, or hemolyzed specimen.

Reference Range:
Negative – No significant level of Legionella pheumophila Type 1-6 IgG antibody detected.
Equivocal – Questionable presence of Legionella pneumophila Type 1-6 IgG antibody detected. Repeat testing in 10-14 days may be helpful.
Positive – Presence of Legionella pneumophila Type 1-6 IgG antibody detected is suggestive of current or past infection.
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time. This assay may detect infection by any of the serotypes 1-6.

PeaceHealth Laboratories