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87070:LEGIONELLA PNEUMOPHILA ANTIBODIY (TYPES 1-6), IgG | |||||||||
Methodology: | Indirect Fluorescent Antibody | ||||||||
Edit Date: | 4/5/2010 | ||||||||
Performed: | Monday-Friday | ||||||||
Released: | 3-5 days after setup at PeaceHealth Laboratories’ reference lab | ||||||||
CPT Code: | 86713-90 | ||||||||
Specimen Collection Details | |||||||||
Collection: | One 7.5 mL serum separator tube (SST). | ||||||||
Handling: | Allow to clot, centrifuge and immediately separate serum into a plastic vial. Mark specimen plainly as “acute” or “convalescent.” | ||||||||
Stability: | 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles. | ||||||||
Standard Volume: | 1 mL serum | ||||||||
Minimum Volume: | 0.5 mL serum | ||||||||
Transport: | Refrigerated. | ||||||||
Comments: | Parallel testing is preferred and convalescent specimen must be received within 30 days of receipt of acute specimen. Acute and convalescent tubes must be marked as such. | ||||||||
Rejection Criteria: | Severely lipemic, contaminated, or hemolyzed specimen. |
Reference Range: | |||||||||||||||||||
<1:128 |
Negative – No significant level of Legionella pheumophila Type 1-6 IgG antibody detected.
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1:128 |
Equivocal – Questionable presence of Legionella pneumophila Type 1-6 IgG antibody detected. Repeat testing in 10-14 days may be helpful.
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≥1:256 |
Positive – Presence of Legionella pneumophila Type 1-6 IgG antibody detected is suggestive of current or past infection.
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Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time. This assay may detect infection by any of the serotypes 1-6. |