2-4 days after setup at Peacehealth Laboratories’ reference lab
Specimen Collection Details
One 7.5 mL serum separator tube (SST).
Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial. Clearly label tube “acute” or “convalescent.”
48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
1 mL serum (pediatric: 200µL)
0.5 mL serum
Parallel testing is preferred and convalescent specimen MUST be received within 30 days from receipt of acute specimen. Cross reactions may occur between Brucella and F. tularensis antigens and antisera; therefore, parallel tests should be run with those antigens. A fourfold rise in titer ≥ 1:160 is considered diagnostic. A single serum titer of 1:160 or 1:320 is suggestive of Brucellosis when accompanied by a compatible clinical course in a patient with a history of potential exposures.
Severely lipemic, contaminated, hemolyzed or heat inactivated specimen.