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Methodology: Bacterial Agglutination
Edit Date: 7/28/2010
Performed: Monday-Friday
Released: 2-4 days after setup at Peacehealth Laboratories’ reference lab
CPT Code: 86622-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial. Clearly label tube “acute” or “convalescent.”
Stability: 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles.
Standard Volume: 1 mL serum (pediatric: 200µL)
Minimum Volume: 0.5 mL serum
Transport: Refrigerated.
Comments: Parallel testing is preferred and convalescent specimen MUST be received within 30 days from receipt of acute specimen. Cross reactions may occur between Brucella and F. tularensis antigens and antisera; therefore, parallel tests should be run with those antigens. A fourfold rise in titer ≥ 1:160 is considered diagnostic. A single serum titer of 1:160 or 1:320 is suggestive of Brucellosis when accompanied by a compatible clinical course in a patient with a history of potential exposures.
Rejection Criteria: Severely lipemic, contaminated, hemolyzed or heat inactivated specimen.

Reference Range:
<1_80 Negative

PeaceHealth Laboratories