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| 93130:AMINOLEVULINIC ACID DEHYDRATASE | |||||||||
| Alias Names: | ALA D / ALA-D / ALAD / Doss Porphyria / PBG (Porphobilinogen) Synthase / Plumboporphyria / Soft-ALAD | ||||||||
| Methodology: | Enzymatic End Point / Spectrofluorometric | ||||||||
| Edit Date: | 8/18/2010 | ||||||||
| Performed: | Monday-Friday | ||||||||
| Released: | 3-4 days after setup at PeaceHealth Laboratories’ reference lab | ||||||||
| CPT Code: | 82657-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 10 mL green top tube (heparin). Also acceptable: One 4 mL lavender top tube (EDTA).
NOTE: Patient should fast 12-14 hours (water only) and abstain from alcohol 24 hours prior to collection. |
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| Handling: | Immediately place on wet ice. Transfer to a 12 mL graduated centrifuge tube and centrifuge 10 minutes at 2000 rpm. Record volume of packed cells. Discard supernatant plasma. Wash erythrocytes 2 times by resuspension in 5.0 mL cold 0.9% saline, discarding supernatant after each washing. Resuspend packed cells to original volume with 0.9% saline. Invert gently to mix. Record volume of packed cells and total volume of erythrocyte suspension on requisition. Freeze. | ||||||||
| Standard Volume: | 5 mL RBC resuspension | ||||||||
| Minimum Volume: | 1 mL RBC resuspension | ||||||||
| Transport: | Frozen. | ||||||||
| Comments: | Useful in confirming a diagnosis of aminolevulinic acid dehydratase deficiency porphyria (ADP). NOTE: This assay re-activates ALAD that has been inhibited by lead, and therefore it is not useful in assessment of lead intoxication. |
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| Rejection Criteria: | Thawed specimen; hemolysis; frozen whole blood. | ||||||||