14 days after setup at PeaceHealth Laboratories’ reference lab
Specimen Collection Details
One 7.5 mL serum separator tube (SST).
Allow to clot, centrifuge and separate serum into a plastic vial. Freeze within 2 hours of collection.
Frozen indefinitely. Unstable ambient or refrigerated.
1 ml serum
0.5 mL serum
Frozen on dry ice.
C3 NeF is an autoantibody that binds to the alternative pathway C3 convertase and prolongs its activity in vivo. Its presence can be detected by mixing the patient’s serum with normal serum and looking for conversion of the C3 in the normal serum by 2-dimensional immunoelectrophoresis under conditions that only allow alternative pathway activation. This assay measures C3 NeF function and is semi-quantitative. Experience has shown that immune complexes do not interfere with the assay.