Print View Attached Docs:
Alias Names: C3 NeF
Methodology: 2-Dimensional Immunoelectrophoresis
Edit Date: 9/15/2010
Performed: Monday-Friday
Released: 14 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 86327-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST).
Handling: Allow to clot, centrifuge and separate serum into a plastic vial. Freeze within 2 hours of collection.
Stability: Frozen indefinitely. Unstable ambient or refrigerated.
Standard Volume: 1 ml serum
Minimum Volume: 0.5 mL serum
Transport: Frozen on dry ice.
Comments: C3 NeF is an autoantibody that binds to the alternative pathway C3 convertase and prolongs its activity in vivo. Its presence can be detected by mixing the patient’s serum with normal serum and looking for conversion of the C3 in the normal serum by 2-dimensional immunoelectrophoresis under conditions that only allow alternative pathway activation. This assay measures C3 NeF function and is semi-quantitative. Experience has shown that immune complexes do not interfere with the assay.

PeaceHealth Laboratories