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| 83370:E. HISTOLYTICA ANTIBODY (AMEBIASIS) | |||||||||
| Alias Names: | Amebiasis (E. Histolytica) Antibody / Entamoeba / Amebiasis / E Histolytica | ||||||||
| Methodology: | Enzyme-Linked Immunosorbent Assay (ELISA) | ||||||||
| Edit Date: | 6/11/2010 | ||||||||
| Performed: | Tuesday, Friday | ||||||||
| Released: | 1-5 days after setup at PeaceHealth Laboratories’ reference lab. | ||||||||
| CPT Code: | 86753-90 | ||||||||
| Specimen Collection Details | |||||||||
| Collection: | One 7.5 mL serum separator tube (SST). | ||||||||
| Handling: | Allow to clot, centrifuge and immediately separate serum. Label specimen as ‘acute’ or convalescent.’ | ||||||||
| Stability: | 48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles. | ||||||||
| Standard Volume: | 1 mL serum. | ||||||||
| Minimum Volume: | 100 µL serum. | ||||||||
| Transport: | Refrigerated. | ||||||||
| Rejection Criteria: | Severly lipemic, contaminated, heat-inactivated, or hemolyzed specimen. | ||||||||
| Interpretive Data IV=Index Value: Optical Density (OD) of the patient serum divided by the OD of the cutoff value. | |||||||||||||||||||
| ≤ 0.79 IV | Negative: No significant level of detectable E. histolytica IgG antibody. | ||||||||||||||||||
| 0.80-1.19 IV | Equivocal: Repeat testing in 10-14 days may be helpful. | ||||||||||||||||||
| ≥ 1.20 IV | Positive: IgG antibody to E. histolytica detected, which may indicate a current or recent infection. | ||||||||||||||||||
| Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.
In the case of extraintestinal complications, a postivie antibody can indicate amebiasis even though stool findings are negative. |
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